Soybean variety APA95-15294

ABSTRACT

The present invention is in the field of soybean variety APA95-15294 breeding and development. The present invention particularly relates to the soybean variety APA95-15294 and its progeny, and methods of making APA95-15294.

THE FIELD OF THE INVENTION

[0001] The present invention is in the field of soybean varietyAPA95-15294 breeding and development. The present invention particularlyrelates to the soybean variety APA95-15294 and its progeny, and methodsof making.

BACKGROUND OF THE INVENTION

[0002] The breeding and development of crops has been ongoing across thelast 1000 years. The pace of this development in soybeans, as an animalfoodstuff and as an oil seed has dramatically increased in the last onehundred years. Planned programs of plant breeding have increased thegrowth, yield and environmental hardiness of the soybean germplasm. Dueto the sexual reproduction traits of the soybean the plant is basicallyself-pollinating. A self pollinating plant permits pollen from oneflower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant. This is a rare occurrence in nature.

[0003] Thus the growth and development of new soybean germplasm requiresintervention by the breeder in the pollination of the soybean. Thebreeders'selections of methods of intervening in the pollination dependon the type of trait that is being selected. Soybeans are developed fora number of different types of traits morphological (form andstructure), phenotypical, for growth habit, daylength temperaturerequirements to initiate floral or reproductive development yield. Thegenetic complexity of the trait drives the breeding method. Backcrossbreeding is employed when the cultivar that is being bred has a fairlyfull profile of desirable traits, but lack one or two traits that arehighly inheritable. Backcrossing is often employed to move diseaseresistance, insect resistance and transgenes (hereinafter DNA which areintroduced into the original ancestor germplasm by a transformationmethod) into other varieties.

[0004] When the variety is being employed to develop a new variety or animproved variety the selection methods include pedigrees, recurrent,modified and mass selection and backcrossing. Each of these selectiontechniques is employed with the efficiency of the breeding procedure asthe driver. The breeding procedure requires a continuous evaluation ofthe success of the breeding program. The success is measured by yieldincrease, commercial appeal and environmental adaptability of thedeveloped germplasm.

[0005] New varieties must be tested thoroughly to compare thedevelopment with the commercially available soybeans. This testingusually requires at least two years and up to six years of comparisonswith other commercial soybeans. Varieties that lack the entire desirablepackage of traits can be used as parents in new populations for furtherselection. The breeding and associated testing process is 8 to 12 years'progression toward a new variety. Thousands of lines are produced andlimited lines are selected in each step of the process. Thus thebreeding system is like a funnel with numerous lines and selections inthe first few years and fewer and fewer lines in the middle years untilone line is selected for the final development testing.

[0006] The selected line or variety will be evaluated for it's thegrowth and development and yield. These traits of a soybean are a resultof the varieties genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since prefect growthconditions do not exist field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

[0007] Selecting for good soybean yield potential under a number ofdifferent environmental conditions is a process that requires planning,analysis of data in a number of seasons. Identification of the varietiesthat carry a superior combination of traits that provides thisconsistent yield potential is a complex science. Other plant traits,unusual weather patterns, diseases, and insect damage often mask thegenotypic traits. One widely employed method of identifying a superiorplant is to observe its performance relative to commercial andexperimental plants in replicated studies. These types of studies givemore certainty to the genetic potential and value of the plant.

[0008] The goal of the soybean plant breeder is to produce unique andnew soybeans and hybrids of the soybeans. To accomplish this the plantbreeder painstakingly crosses two or more varieties or germplasm. Thenthe results of this cross is repeatedly selfed or backcrossed to producenew genetic patterns. Additionally, the breeder can introduce mutationsinto the genetic material. These can alter herbicide resistance, fattyacid compositions, and amino acid compositions of the seeds and thelike. Fortunately, through transformation in combination with breedingthe plant breeder can alter or introduce some limited alleles into thebreeding material. This capability is widening the potential uses andmarkets for the various products and by products of oil seed plants suchas soybean. One of the products of soybeans is the oil of the seed.Soybean oil is employed in a number of retail products. Soybean meal isalso used in food and animal feedstuffs. The traits a breeder selectsfor can be driven by the ultimate goals of the end user of the product.Thus if the goals of the end user is to produce an oil with a high levelof oleic acid and a lower level of linoleic acid then the breeder maydrive the genetics toward levels of fatty acids and accept some lesseryield potentials or other less desirable agronomic traits.

[0009] Regardless of the market characteristics of the plant mostbreeding proceeds along a similar path on a yearly basis. The breederannually selects the germplasm to advance on into further development.This germplasm is grown in different locations at different altitudes,in different climates and subjected to different soil conditions basedon the datum collected individual plants are selected during the end ofthe growing season. Due to the number of genes within each chromosomemillions of genetic combinations exist in the breeders experimentalmaterial. This genetic diversity is so vast that a breeder cannotproduce the same two cultivars twice using the exact same material. Thusthe developing a single variety of useful commercial soybean germplasmis highly unpredictable, and requires intensive research.

[0010] The development of new soybeans comes through breeding techniquessuch as recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure that is used to save labor costs.Other breeding methods are taught in several soybean textbooks.

[0011] The development of soybean cultivars most often requires thedevelopment of hybrid crosses (the exception being initial developmentof mutants directly through the use of the mutating agent ortransformants directly through transformation methods) and the selectionof progeny therefrom. Hybrids can be achieved by manual manipulation ofthe sexual organs of the soybean or by the use of male sterilitysystems. The breeder attempts to identify true hybrids by a readilyidentifiable trait. These hybrids are then selected and repeatedlyselfed and selected to form new homozygous lines from the heterozygoushybrids.

[0012] Outcrossing to a number of different parents creates breedingpopulations of fairly heterozygous populations. These populations areproduced and used in pedigree breeding and recurrent selection. Pedigreebreeding is commonly used with two parents which possess favorable,complementary traits. The parents are crossed to form a F1 hybrid. Theprogeny of the F1 hybrid is selected from this the best individuals F2are selected; this is repeated in the F3 and F4 generations. Theinbreeding is carried forward and at F5-F7 the best lines are selectedand tested in the development stage for potential usefulness.

[0013] Mass and recurrent selection can be used to improve populations.Several parents are intercrossed and plants are selected based onselected characteristics like superiority or excellent progeny.

[0014] In backcross breeding a genetic allele or loci is transferredinto a desirable homozygous recurrent parent. The trait is in the donorparent and is tracked into the recurrent parent. The resultant plant islike the recurrent parent with the new desired allele or loci.

[0015] The single-seed descent method involves use of a segregatingplant population for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

[0016] Soybean Glycine max (L) is an important oil seed crop and avaluable field crop.

SUMMARY OF THE INVENTION

[0017] One embodiment of the invention relates to seed of a soybeancultivar designated APA95-15294. And the plant, or parts includingovule, a tissue culture of regenerable cells, cells or protoplasts beingfrom a tissue selected from the group consisting of leaves, pollen,embryos, meristematic cells, roots, root tips, anthers, flowers, seeds,stems and pods and pollen thereof, produced by growing the seed.

[0018] The invention in one aspect covers a soybean plant, or partsthereof, having all of the physiological and morphologicalcharacteristics of the soybean plant.

[0019] Another aspect of this invention is the soybean plant seed orderived progeny which contains a transgene (which include DNA whether ornot it is a full or partial sequence) affecting herbicide resistance,insect resistance, resistance to disease, nematodes, male sterility, andaltered oil or amino acids or other nutritional quality.

[0020] The present invention further covers a method for producing asoybean seed with the steps of crossing at least two parent soybeanplants and harvesting the hybrid soybean seed, wherein at least oneparent soybean plant is the present invention. In another aspect of theinvention covers the hybrid soybean seed and the progeny soybean plantand resultant seed, or parts thereof from the hybrid seed or plant orits progeny.

[0021] In an additional aspect the invention covers a method forproducing a soybean progeny from the invention by crossing soybean lineAPA95-15294 with a second soybean plant to yield progeny soybean seedand then growing progeny soybean seed to develop a derived soybean line.

[0022] Yet another aspect of the invention covers a method for abreeding program using plant breeding techniques which employ thesoybean plant APA95-15294 as plant breeding material and performingbreeding by selection techniques, backcrossing, pedigree breeding,marker enhanced selection, mutation and transformation.

DETAILED DESCRIPTION Soybean Data Definitions

[0023] Following is a series of definitions of traits that are collectedas part of the research yield trial program. TRAIT DESCRIPTION TIMINGSCALE Core Traits - HILA COLOR (HC) Phenotypic observation Packaging G =Gray Br = Brown Ib = Imperfect Black Bl = Black Y = Yellow FLOWER COLOR(FC) Phenotypic observation R1 W = White (Beginning P = Purple Bloom) X= Mix POD COLOR (POD) Phenotypic observation R8 (Full T = Tan Maturity)B = Brown X = Mixed PUBESCENCE COLOR Phenotypic observation R8 (Full G =Gray (PUB) Maturity) T = Tawny Lt = Tawny X = Mixed Core Traits - Takenon all Yield Trial Sets Included in harvest GWT Grain weight/plotHarvest Pounds H2O Grain moisture/plot Harvest % moisture MATURITY (MAT)# of days after Aug. 31 R8 (Full Taken in days after Aug. 31 Taken onall reps at 6 when 95% of the main Maturity) locations. stem pods in theplot have reached their mature color PLANT HEIGHT (HT) The averagemeasured Harvest Taken in inches Taken on all reps at 4 plot height.locations where most growth occurs. STEM LODGING (LODGE) Taken whereverdifferential lodging occurs Rating based on the angle of the majority ofplants in the plot relative to the ground at 0° Harvest 1 = All erect 2= 67°3 = 45°4 = 22°5 = 0°

BRANCHING Rating of the number of Harvest 1 = stick - no branch (BRANCH)branches and their 2 = 1-2 branches relative importance to 3 = 2-3branches-average yield. Taken at growth 4 = >3-4 branches expressivelocations 5 = >5 branches - profuse SHATTER (SHAT) Rating of pre-harvestHarvest 1 = <1% of plot shattered Taken on tests where loses based on 2= 1-10% shattered shattering occurs percentage of plants 3 = 10-20%shattered with open pods 4 = 20-30% shattered 5 = >30% shatteredOptional Traits EMERGENCE (EMG) Rating of the uniform When SeedlingStages: establishment of 50% or 1 = V1 (Uniform), seedling more ofStand>85% the plants 2 = VC&V1 (less uniform), have reached Stand>85% V1(First 3 = VE&VC&V1, (variable) leaf node) Stand>75% VE (emergence) 4 =Gaps>1′, Stand<75% VC (cotyledon 5 = Gaps>2′, Stand<=50% stage) GREENLODGING (GLODGE) Rating based on the angle of the majority of plants inthe plot R5 to R6 (Beginning seed to Full seed) 1 = All erect 2 = 67°3 =45°4 = 22°5 = 0°

Disease/Stress Traits PHYTOPHTHORA The observed reaction Field 1 to 5 (1= best) ROOT ROT of the variety in the Nursery or (PFT) presence of theYT (Yield organism Trial) plots BROWN STEM ROT The observed reactionField 1 to 5 (1 = best) (BSR) of the variety in the Nursery or presenceof the YT plots organism SCLEROTINIA WHITE The observed reaction Field 1to 5 (1 = best) MOLD of the variety in the Nursery or (SWM) presence ofthe YT plots organism SUDDEN DEATH The observed reaction Field 1 to 5 (1= best) SYNDROME of the variety in the Nursery or (SDS) presence of theYT plots organism STEM CANKER The observed reaction Field 1 to 5 (1 =best) (STEMC) of the variety in the Nursery or presence of the YT plotsorganism CHARCOAL ROT The observed reaction Field 1 to 5 (1 = best)(CHROT) of the variety in the Nursery or presence of the YT plotsorganism FROG EYE The observed reaction Field 1 to 5 (1 = best) (FRGEYE)of the variety in the Nursery or presence of the YT plots organism IRONDEFICIENCY A composite rating of Hill Plots 1 to 5 (1 = best) CHLOROSISYellow Flash, Green- in soils of (IDC) up, and Stunting in 7.9 pH orHpH(high pH) soil higher

[0024] Definitions of Staging of Development

[0025] The plant development staging system employed divides stages asvegetative (V) and reproductive (R). This system accurately identifiesthe stages of a soybean plant. However, all plants in a given field willnot be in the stage at the same time. Each specific V or R stage isdefined as when 50% or more of the plants in the field are in or beyondthat stage.

[0026] The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n) The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=Full Seed; R7=beginningmaturity; R8=Full maturity.

[0027] BROWN STEM ROT (BSR)—This disease is caused by the fungusPhialophora gregata. The disease is a late-season, cool-temperature,soilborne which in appropriate favorable weather can cause up to 30percent yield losses in soybean fields.

[0028] SUDDEN DEATH SYNDROME (SDS)—This disease is caused byslow-growing strains of Fursarium solani that produce bluish pigments inculture. The disease is mid- to late season soil borne and occurs insoybean fields with high yield potential. Yield losses may be total Iseverely affected fields.

[0029] SOYBEAN CYST NEMATODE—The Soybean Cyst Nematode (SCN) Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans.

[0030] MATURITY DATE. Plants are considered mature when 95% of the podshave reached their mature color. The number of days are eithercalculated from Sep. 1 or from the planting date.

[0031] RELATIVE MATURITY GROUP(RM). Industry Standard for varietiesgroups, based day length or latitude. Long day length (northern areas inthe Northern Hemisphere) are classified as (Groups 000,00,0,) and extendto very short day lengths variety groups (southern areas in NorthernHemisphere) classified as (Groups VII,VIII, IX).

[0032] SEED YIELD (Bushels/Acre). The yield in bushels/acre is theactual yield of the grain at harvest.

[0033] SHATTERING. The rate of pod dehiscence prior to harvest. Poddehiscence involves beans dropping out of the pods.

[0034] PLANT. Means the plant cells, plant protoplasts, plant cells oftissue culture from which soybean plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof plants, such as pollen, nodes, roots, flowers, seeds, pods, leaves,stems, and the like.

[0035] The present invention is APA95-15294. This soybean is developedfor its use of the beans. The maturity group for this soybean is groupII. But this soybean is much closer to a long day length group III thana long day length group I. The traits of the invention are listed below.TRAIT RM 2.8 HR Flower Color purple Pubescene Color Gray Pod Color TanHila Color Yellow/BF Seed/Lb 2800-3100 Lust Perox Phytophthora Root Rot2.1 Rkn Brown Stem Rot 4.0 Iron Deficiency Chlorosis 4.1 SclerotiniaWhite Mold Sudden Death Syndrome Stem Canker Charcoal Rot Frog Eye SCNPGR

[0036] The instant invention provides methods and composition relatingto plants, seeds and derivatives of the soybean cultivar APA95-15294.Soybean cultivar APA95-15294 has superior characteristics. TheAPA95-15294 line has been selfed sufficient number of generations toprovide a stable and uniform plant variety.

[0037] Cultivar APA95-15294 shows no variants other than expected due toenvironment or that normally would occur for almost any characteristicduring the course of repeated sexual reproduction. Some of the criteriaused to select in various generations include: seed yield, lodgingresistance, emergence, appearance, disease tolerance, maturity, plantheight, maturity and shattering data.

[0038] The inventor believes that APA95-15294 is similar to thecomparison varieties. However, as shown in the tables, APA95-15294differs from these cultivars.

[0039] Direct comparisons were made between APA95-15294 and thesecompeting commercial varieties. Traits measured included yield,maturity, moisture, lodging, plant height, field emergence. The resultsof the comparison are presented in below. The number of tests in whichthe varieties were compared is shown. The deviation or difference of theresults, T-value and the traits which showed a significant differenceand the level of that significance are also in this table. The presentinvention APA95-15294 is employed in a trialling for a number ofcharacteristics. These test allow the usefulness of the invention to beshown in light of the environmental-genetic interactions.

[0040] The present invention is carrying better yield at a significantdifference level and better moisture than the line D 285. Additionally,the present invention has less tendency to lodge or branch. Ent YldMoist Appearance Branch BSR E Lg Mat Pltht PRR Shatter IDC APA95-15294 60.6  10.9  2.4  2.7 2.0  1.3 17.7 35.3  1.1 D285  55.9  11.3  2.9  3.32.0  1.9 20.7 35.6  1.0 # REPS 115 116 10 13 0 6 65 39 49 0 18 0 Diff 4.7 −0.4 −0.5 −0.6 0.0 −0.6 −3.0 −0.3  0.1 Std  9.0  1.0  0.7  0.8 0.6 0.8  3.3  5.3  0.2 T-val  5.59 −4.59 −2.24 −2.89 0.00  6.22  5.79 −0.40 1.00 Prob  0.000***  0.000***  0.052***  0.014** *** 1.00  0.000*** 0.000***  0.690 ***  0.331 ***

[0041] The present invention APA95-15294 is showing significantly betteryield and significantly less tendency to shatter than is D260.Additionally, the present invention carries less moisture in the seed atthe time of harvest than does D260 though not necessarily at asignificant difference level. Ent Yld Moist Appearance Branch BSR E LgMat Pltht PRR Shatter IDC APA95-15294  60.5  10.9  2.4  2.6 2.0  1.318.0 35.3  1.1 D260  55.9  11.4  2.6  2.6 1.8  1.4 16.4 32.2  1.6 # REPS112 113 10 14 0 6 63 40 49 0 18 0 Diff  4.6 −0.5 −0.2  0.0 0.2 −0.1  1.7 3.1 −0.6 Std  7.4  5.0  0.6  1.0 0.8  0.6  2.9  4.  1.3 T-val  6.61−0.97 −1.00  0.00 0.54  0.89  3.57  5.15 −1.82 Prob  0.000***  0.335 0.343  1.000 *** 1.611  0.375  0.001***  0.000*** ***  0.086* ***

[0042] In comparsion with this commercial line the present inventionshows both lower moisture and better significantly different yield.Additionally, the present invention is less prone to lodging than is thecommercial line.

[0043] This invention also is directed to methods for producing asoybean plant by crossing a first parent plant with a second parentplant wherein the first or second parent plant is present invention or aderived progeny therefrom. Further, both first and second parent plantscan come from the soybean line APA95-15294. Crossing a self pollinatingplant can be difficult. The female flower can be emasculated if desiredand manual pollination is accomplished by removing the stamens andpistil from a flower and brushing the anthers against the stigma. Accessis gained to the stamens by piercing the keel with tweezers and allowingthem to open to push the petals away. Brush the anthers on the stigma.When pollen is on the stigma pollination should occur.

[0044] Genetic male sterility is available in soybeans. This isparticularly useful for recurrent selection programs.

[0045] A variety of breeding methods can be selected depending on themode of reproduction, the trait, the condition of the germplasm. Thus,any such methods using the APA95-15294 are part of this invention:selfing, backcrosses, recurrent selection, mass selection and the like.

[0046] Other means of introducing traits in to the APA95-15294 varietycan be indirectly or directly through biotechnology. The APA95-15294variety may be converted usually be backcrossing to have an inheritedtransgene. One common example of these transgenes is a mutated EPSPSencoding gene, such as taught in the U.S. Pat. No. 4,535,060. Thistransgene provides herbicide resistance. When this transgene is presentthe plant may be directly treated with the herbicide glyphosate withoutthe result of significant damage to the plant. This phenotype providesthe field with stand on the benefit of control of weed growth in a fieldwith the herbicide glyphosate. It will be understood to those of skillin art that other transgenes can be used for herbicide resistance suchas the imazethapyr tolerant (IT or IR™) gene, STS (™Dupont), aglufosinate resistant gene, and a paraquat resistant gene for othertypes of herbicide affects. For example a gene providing the oxalateoxidase enzyme. Such a gene is listed in U.S. Pat. No. 6,187,511. Theoxalate oxidase enzyme in combination with a gene encoding for theoxalate decarboxylate (See U.S. Pat. No. 5,547,870) or the ox decarboxalone can provide disease resistance. These genes can be used to provideSclerotonia resistance plants. Alternatively transgenes can be used toprovide altered oil or other nutritional affects.

[0047] Numerous other transgenes can be introduced directly bytransformation methods such as by microparticle bombardment, PEGtransformation, sonification and the like. Transformation can beperformed on explants, cell suspensions, protoplasts or meristem orother regenerable plant cells thereafter the transformant can beemployed to introduce the transgene into the invention by standardbreeding practices or by enhanced marker assisted breeding practices.Transformation methods are means for integrating new genetic codingsequences (transgenes) into the plant's genome by the incorporation ofthese sequences into a plant through man's assistance. Many dicotsincluding soybeans can easily be transformed with Agrobacterium. Themost common method of transformation after the use of Agrobacterium isreferred to as gunning or microprojectile bombardment. This process hassmall gold-coated particles coated with DNA (including the transgene)shot into the transformable material. Techniques for gunning DNA intocells, tissue, explants, zygotes, meristems, callus, embryos, and thelike are well known in the prior art. The DNA used for transformation ofthese plants clearly may be circular, linear, and double or singlestranded. Usually, the DNA is in the form of a plasmid. The plasmidusually contains regulatory and/or targeting sequences which assists theexpression of the gene in the plant. The methods of forming plasmids fortransformation are known in the art. Plasmid components can include suchitems as: leader sequences, transit polypeptides, promoters,terminators, genes, introns, marker genes, etc. The structures of thegene orientations can be sense, antisense, partial antisense, or partialsense: multiple gene copies can be used. After the transformation of theplant material is complete, the next step is identifying the cells ormaterial, which has been transformed. In some cases, a screenable markeris employed such as the beta-glucuronidase gene of the uidA locus of E.coli. Then, the transformed cells expressing the colored protein areselected for either regeneration or further use. In many cases, aselectable marker identifies the transformed material. The putativelytransformed material is exposed to a toxic agent at varyingconcentrations. The cells not transformed with the selectable marker,which provides resistance to this toxic agent, die. Cells or tissuescontaining the resistant selectable marker generally proliferate. It hasbeen noted that although selectable markers protect the cells from someof the toxic affects of the herbicide or antibiotic, the cells may stillbe slightly effected by the toxic agent by having slower growth rates.If the transformed material was cell lines then these lines areregenerated into plants. The cells' lines are treated to induce tissuedifferentiation. Methods of regeneration of cellular are well known inthe art. The plants from the transformation process or the plantsresulting from a cross using a transformed line or the progeny of suchplants are transgenic plants that carry the transgene.

[0048] Deposit Information

[0049] A deposit of the Advanta USA Inc. Seed soybean cultivarAPA95-15294 disclosed above and recited in the appended claims will bemade with the American Type Culture Collection (ATCC), 10801 UniversityBoulevard, Manassas, Va.

[0050] 20110. The date of deposit was XXXX. The deposit of 2,500 seedsmaintained by Advanta USA Inc. since prior to the filing date of thisapplication. All restrictions upon the deposit have been removed, andthe deposit is intended to meet all of the requirements of 37 C.F.R..sctn.1.801-1.809. The ATCC accession number is XXX. The deposit will bemaintained in the depository for a period of 30 years, or 5 years afterthe last request, or for the effective life of the patent, whichever islonger, and will be replaced as necessary during that period.

[0051] Accordingly, the present invention has been described with somedegree of particularity directed to the preferred embodiment of thepresent invention. It should be appreciated, though, that the presentinvention is defined by the following claims construed in light of theprior art so that modifications or changes may be made to the preferredembodiment of the present invention without departing from the inventiveconcepts contained herein.

What is claimed is:
 1. A soybean seed designated APA95-15294, a sampleof said seed deposited under ATCC Accession No. XXXXX.
 2. A plant, orparts thereof, produced by growing the seed of claim
 1. 3. Pollen of theplant of claim
 2. 4. Oil of the seed of claim
 1. 5. A soybean plant, orparts thereof, having all of the physiological and morphologicalcharacteristics of the soybean plant of claim
 2. 6. A tissue culture ofregenerable cells of a soybean plant of cultivar APA95-15294, whereinthe tissue regenerates plants capable of expressing all of themorphological and physiological characteristics of the cultivarAPA95-15294.
 7. A tissue culture according to claim 6, the cells orprotoplasts being from a tissue selected from the group consisting ofleaves, pollen, embryos, meristematic cells, roots, root tips, anthers,flowers, seeds, stems and pods.
 8. A soybean plant regenerated from thetissue culture of claim 6, capable of expressing all of themorphological and physiological characteristics of soybean cultivarAPA95-15294.
 9. A method for producing a soybean seed comprisingcrossing at least two soybean plants and harvesting the resultantsoybean seed, wherein said at least one soybean plant is the soybeanplant of claim
 2. 10. A soybean plant, or parts thereof, produced bygrowing said soybean seed of claim
 9. 11. Soybean seed comprising saidsoybean plant of claim 10 as at least one of its ancestors.
 12. A methodfor producing a hybrid soybean seed comprising crossing a soybean plantaccording to claim 2 with a second soybean plant.
 13. Soybean seedcomprising as one of its ancestors said hybrid soybean plant of claim12.
 14. A hybrid soybean plant, or parts thereof, produced by growingsaid hybrid soybean seed of claim
 13. 15. A method for producing aAPA95-15294-derived soybean plant, comprising: a) crossing soybean lineAPA95-15294 with a second soybean plant to yield progeny soybean seed;b) growing said progeny soybean seed to yield said APA95-15294-derivedsoybean plant.
 16. A method according to claim 15 wherein step c isharvesting the progeny soybean seed from the derived soybean plant andselecting progeny seed therefrom capable of forming anotherAPA95-15294-derived soybean plant, and step d is repeating Step (c) atleast once.
 17. A method for developing a soybean plant in a soybeanplant breeding program using plant breeding techniques which includeemploying a soybean plant as a source of plant breeding materialcomprising: using the soybean plant of claim 2 and wherein said plantbreeding techniques are selected from the group consisting of: recurrentselection, backcrossing, pedigree breeding, genetic marker enhancedselection, and transformation.
 18. A method of claim 9 with the step ofselecting at least one of the two soybean plants to have at least onetransgene.
 19. The method of claim 18 wherein the transgene is selectedfrom the group consisting of herbicide resistance, insect resistance,resistance to disease, male sterility, and altered oil, amino acid,nutritional quality, IMI resistant gene, glyphosate resistant gene, theoxalate oxidase encoding gene, the oxalate decarboxylate encoding gene.